Fluorescence spectroscopy characteristics of Indonesian citrus

Abstract

Citrus suffering physical damage to the peel would spread polymethoxylated flavone that can be detected by its spectroscopic characteristics. Fluorescence spectroscopy is expected to offer an important means for physical damage detection of Indonesian citrus. This study was conducted to determine the characteristics of fluorescence spectra such as absorbance and fluorescence spectra of four varieties of citrus from Indonesia and identified the fluorescence substance in citrus peels. From this study, the absorbance and fluorescence wavelengths of Indonesian citrus were found to vary from 315 nm to 340 nm and from 500 nm to 555 nm respectively. The fluorescence substances were identified as polymethoxylated flavone such as hesperidin, tangeretin, and nobiletin. The fluorescence spectroscopy could be used to detect defects in citrus peel.