Effect of ammonia on Na + transport across isolated rumen epithelium of sheep is diet dependent

Abstract

The cellular uptake of ammonia affects the intracellular pH (pH i ) of polar and non-polar cells. A predominant uptake of NH 3 and its intra-cellular protonation tend to alkalinise the cytoplasm, whereas a predominant uptake of NH 4 + acidifies the cytoplasm by reversing this reaction. Hence, the well-known absorption of ammonia across the rumen epithelium probably causes a change in the pH i . The magnitude and direction of this change in pH i (acid or alkaline) depends on the relative transport rates of NH 3 and NH 4 + . Consequently, the intra-cellular availability of protons will influence the activity of the Na + -H + exchanger, which could affect transepithelial Na + transport. The aim of the present study has been to test this possible interaction between ruminal ammonia concentrations and Na + transport. The term ammonia is used to designate the sum of the protonated (NH 4 + ) and unprotonated (NH 3 ) forms. Isolated ruminal epithelium of sheep was investigated by using the Ussing-chamber technique in vitro. The present results indicate that ammonia inhibits Na + transport across the rumen epithelium of hay-fed sheep, probably by binding intracellular protons and thus inhibiting Na + -H + exchange. By contrast, ammonia stimulates Na + transport in concentrate-fed and urea-fed sheep, which develop an adaptation mechanism in the form of an increased metabolism of ammonia in the rumen mucosa and/or an increased permeability of rumen epithelium to the charged ammonium ion (NH 4 + ). Intracellular dissociation of NH 4 + increases the availability of protons, which stimulate Na + –H + exchange. This positive effect of ruminal ammonia on Na + absorption may significantly contribute to the regulation of osmotic pressure of the ruminal fluid, because intraruminal ammonia concentrations up to 40 mmol/l have been reported.