microRNA-103 promotes LPS-induced inflammatory injury by targeting c-Myc in HK-2 cells

Abstract

Aims: microRNAs (miRNAs) occupy a vital position in diseases. This research aims to detect miR-103 influence on LPS-resulted HK-2 cells inflammation damage. Methods: Cells were exposed to LPS for inducing injury. CCK-8 and flow cytometry were introduced to detect cell viability and apoptosis rate, respectively. ELISA, qRT-PCR and western blot were respectively used to explore inflammation factors production and relative proteins expression. The relationship between miR-103 and c-Myc was analysed through luciferase reporter assay. Results: LPS led to significant inhibition of cell viability (p