Effects of different NaOCl concentrations followed by 17% EDTA on dentin permeability

Abstract

Aim: The aim of this study is to evaluate the permeability of root dentin after immersion in sodium hypochlorite (NaOCl) at different concentrations for 30 minutes and a final wash with 17% ethylenediaminetetraacetic acid (EDTA) for 3 minutes. Materials and methods: Twenty 1 × 5 mm dentin fragments from the middle third of the root were prepared from 10 bovine teeth and divided into four groups; three of these groups were immersed in 1%, 2.5%, or 5.25% NaOCl for 30 minutes, while group IV was immersed in the saline solution. All dentin fragments were subjected to a final wash with 17% EDTA for 3 minutes. The fluid transport study model was used to measure the fluid conductance induced by hydrostatic pressure. The measurements were repeated twice to confirm the values, and in the case of a discrepancy, the procedure was performed again. Results: The control group (saline solution) presented lower hydraulic conductance mean values with (0.25 ± 0.12). The 5.25% NaOCl followed by 17% EDTA produced the highest mean conductance value (1.18 ± 0.18) followed by 2.5% NaOCl and 17% EDTA with (0.81 ± 0.09) and (0.48 ± 0.02), respectively. The results of the hydraulic conductance evaluation of dentin were different for each NaOCl concentration. After data analyses, there were significant differences from analysis of variance (ANOVA) between all the groups (p < 0.0001). Conclusion: The NaOCl solution concentration affects dentin permeability.