Laser-Based indirect fluorometric detection and quantification in thin-layer chromatography

Abstract

A laser-based Indirect fluorometric detection method for thin-layer chromatography Is described. The new technique can be easily used for quantitative measurements because of its two-dimensional scanning capability. Since It Is based on the indirect fluorescence mode, universal detection is possible without derivatization. Also, It takes only 35s for acquiring a data array of 256 X 64 points with this technique to achieve a detection limit of 6 pg. This Is 100 times lower than when the human eye Is used as a detector for the same samples based on Indirect fluorometry. The linearity in indirect fluorometric detection is found to be over 2 orders of magnitude. © 1989, American Chemical Society. All rights reserved.