Modulation of hyperthermia-induced platelet aggregation inhibition in the presence of urea

Abstract

Purpose: This study has been conducted to evaluate the effect of urea on aggregation responses of heat-treated platelets. Materials and methods: The urea was added to platelet-rich plasma (PRP) samples in final concentrations of 50 and 100mM. PRP samples, with or without exogenous urea, were incubated at 37°C, 39°C and 41°C for 90min and then were stimulated with adenosine diphosphate (ADP) or epinephrine for measuring of platelet aggregation responses. The average reduction in aggregability of heat-treated samples with reference to mean value obtained for control samples treated at 37°C was expressed as inhibition percentage. Results: Aggregation responses of the samples treated in the presence of 50mM and 100mM urea were significantly less inhibited by hyperthermia treatments compared with those treated without exogenous urea. Conclusion: The results indicate that the inhibitory effect of hyperthermia on platelet aggregation responses could be significantly modulated by urea. © 2013 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.