ContEst: Estimating cross-contamination of human samples in next-generation sequencing data

Abstract

Summary: Here, we present ContEst, a tool for estimating the level of cross-individual contamination in next-generation sequencing data. We demonstrate the accuracy of ContEst across a range of contamination levels, sources and read depths using sequencing data mixed in silico at known concentrations. We applied our tool to published cancer sequencing datasets and report their estimated contamination levels. © The Author 2011. Published by Oxford University Press. All rights reserved.