Atividade vasodilatadora in vitro de espécies de Ouratea (Ochnaceae) e de frações de Ouratea semiserrata (Mart.) Engl.

Abstract

Ouratea species occur in the cerrado vegetation and some species are popularly used as tonic, astringent and anti-inflammatory. In the present work, we assayed the vasodilator effects of O. castanaefolia, O. spectabilis and O. semiserrata hydroethanolic extracts in aortic rings precontracted with phenylephrine. Only O. semiserrata stem extract (OS) produced significant vasodilatation (63 ± 3 %, n = 6), at the concentration of 100 mg/ml. Bioguided fractionation of OS afforded a proanthocyanidin-rich fraction that produced 100 ± 5% vasodilatation (n = 6, concentration of 100 mg/ml). The total proanthocyanidin contents were assayed for OS and for the bioactive fractions using a spectroscopic method. Higher proanthocyanidin contents implied in increased vasodilator activity (r2 = 0.9760), suggesting that compounds of this class may be regarded as responsible for the activity of O. semiserrata stem extract.