Comparative study of degradation of reactive dyes and decolorization and detoxification in aqueous solution applying DyP peroxidases isolated from saccharomonospora viridis (Svi DyP) and Thermobifida fusca (Tfu Dyp)

Abstract

The wastewater that originates from the widespread usage of synthetic dyes in the industry have become a severe environmental problem. Several efforts have been made to develop new types of treatment which are capable of performing the degradation of the dyes from the environment. Within this scope, much attention has been drawn to enzymatic approaches, mainly the ones applying oxidative enzymes, such as peroxidases. A recently discovered superfamily of peroxidases, the so called dye-decolorizing peroxidases (DyPs) is a promising alternative to further improve the efficiency of these processes. In this work, two of these peroxidases (Saccharomonospora viridis (SviDyP) and Thermobifida fusca (TfuDyp)) were tested together with twelve different reactive dyes in order to evaluate the efficiency of degradation and decolorization, leading to good results. When applying the SviDyP enzyme in experiments carried out for 12 h in pH 3, the degradation efficiencies were above 80% for some dyes. The biodegradation efficiency data and cyclic voltammograms were recorded to obtain the redox potential of the chosen dyes and enzymes. In addition, an electrochemical biosensor was used to gauge the genotoxicity of the generated bioproducts. This analysis showed that bioproducts from dye degradation mostly present a lower degree of genotoxicity when compared to the control reactions.