Oil cakes as substrate for improved lipase production in solid state fermentation

Abstract

Solid-state fermentation for lipase production from Rhizopus oryzae KG-10, using different low cost available oil cakes JOC (Jatropha oil cake), TOC (Teesi oil cake), MOC (Mustard oil cake), GOC (Groundnut oil cake) was carried and it was found that the fungus produced significant amount of lipase utilizing oil cakes as substrate. Among the four substrates used crude enzyme extracted from MOC medium showed highest activity of 170 IU. Activity of enzyme extracted from medium containing JOC, TOC and GOC were assayed to be 80 IU, 60 IU and 60 IU respectively. Total protein of the crude enzyme extracted from the different medium was estimated by Lowry’s method. Total protein content of extracts from MOC, JOC, TOC and GOC medium were 32 mg/ml, 30 mg/ml, 31.2 mg/ml and 26.4 mg/ml respectively. Thus it could be seen that in about same amount of extracellular proteins the activity was maximum in case of MOC, suggesting it to be the best substrate. Key words – Lipase, Rhizopus oryzae, oil cakes, solid-state fermentation