Antibacterial Activity of Endophytic Bacterial Extracts Isolated from Pineapple Peel (Ananas comosus L.)

Abstract

Endophytic bacteria are known to contain various bioactive substances. Pineapple peel (Ananas comosus L.) is a waste product utilized in environmental preservation. The present study is focused on the isolation of endophytic bacteria from pineapple peel, and the assessment of their antibacterial activity. The ethyl acetate extracts of isolated bacteria were tested for their antibacterial activity using the disc diffusion method. The components of the bacterial extract were identified by UPLC-MS/MS analysis. Extract of the endophytic bacteria (Bacillus velezensis WSM-1) had the highest activity with an inhibition zone diameter of 17.71 mm and 15.87 mm against Staphylococcus aureus and Escherichia coli, respectively. UPLC MS/MS analysis identified several compounds, including; 6-Methoxyquinoline, 2-(6-amino-9H-purin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol, Aklavin, Maltotriose, Cholestanetriol, Piperic acid, Isoflavone, Paromomycin, and Mycaloide B. Paromomycin is known to have antibacterial activity. It is a type of antibiotic that belongs to the aminoglycoside class and is commonly used to treat infections caused by gram-negative bacteria. The results of the present study suggest that the endophytic bacterial isolate from pineapple peel have potential antibacterial activity and there is a need for further study to isolate and characterize the antibacterial active constituents.