FAST START-UP MARINE ANAMMOX PROCESS USING INTENSIVE SHRIMP POND SOLID WASTE AS INOCULUM IN FILTER BIOREACTOR

Abstract

Intensification of shrimp culture increases nitrogen waste in the environment, which impacts decreasing environmental carrying capacity and water quality. The revolutionary discovery of bacteria anaerobic ammonium oxidation (anammox) is a potential and economical strategy for nitrogen removal. This study aimed to determine the performance of nitrogen removal with different inoculums in two bioreactor filters (FtBR). The starter used was intensive shrimp pond waste in Reactor 1 and the addition of anammox bacterial inoculum with intensive shrimp pond waste in Reactor 2. The substrate used was artificial waste containing ammonium and nitrite with concentrations of 70–100 mg-N/L and sterile seawater with a 24-h HRT setting for 120 days. The maximum nitrogen removal performance obtained in this activity with parameters NRR, ACE, and NRE for reactors 1 and 2 was 0.12 kg-N/m3.h, 82.48%, 72.58% and 0.10 kg-N/m3.h, 83.06%, 63, 59%. The stoichiometric ratio of nitrogen in Reactor 1 was 1:1.40:0.12 and in Reactor 2 was 1:1.13:0.14. This value was similar to the stoichiometry of the anammox process. Based on this study, anammox appears to play a role in nitrogen removal and can be a profitable alternative in intensive shrimp pond nitrogen waste treatment.