Application of Antifreeze Proteins for Sperm Cryopreservation

Abstract

Cryopreservation is an important technique used to store various types of cells, tissues, and organs at very low temperature, usually in liquid nitrogen (-196 °C) [1]. However, freezing and thawing process causes cryo-injuries to cells. During cryopreservation creation of extracellular ice may cause rupture of the cell membrane and recrystallization of intracellular and extracellular ice may further damage the cells during the freezing and thawing process, respectively.