Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method

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Abstract

Simian rotavirus SA-11, experimentally seeded, was recovered from raw domestic sewage by a two-step concentration procedure, using filtration through a positively charged microporous filter (Zeta Plus 60 S) followed by ultracentrifugation, effecting an 8,000-fold concentration. By this method, a mean recovery of 81% ± 7.5 of the SA-11 virus, was achieved.

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Mehnert, D. U., Stewien, K. E., Hársi, C. M., Queiroz, A. P. S., Candeias, J. M. G., & Candeias, J. A. N. (1997). Detection of Rotavirus in Sewage and Creek Water: Efficiency of the Concentration Method. Memorias Do Instituto Oswaldo Cruz, 92(1), 97–100. https://doi.org/10.1590/S0074-02761997000100020

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