Correlation of antisperm antibodies with trace elements in seminal fluid of immunologic infertile men

Abstract

Immunological cause may contribute to 5-15% of the male infertility factors, including cryptorchidism, primary testicular failure, testicular trauma, epididymitis, varicocele, idiopathic infertility, and infections. Therefore, ASA can be found in primary or secondary infertile men. The aim is to study the correlation of ASA with trace elements in primary and secondary infertile men. The study was carried out in the Department of Medical Laboratory Technology in Southern Technical University. Seminal fluids were collected from each of them, seminogram test (semen analysis) was performed for each participant, and all semen samples were collected following (3-5) days of abstinence. After liquefaction, sperm concentration, total sperm count, morphology, motility grades were determined using World Health Organization (WHO) standard procedures. The mean values of were significantly increased ASA in infertile men as compared to fertile control group (p<0.0001), sperm concentration significant decrease in infertile men as compared to control group (p<0.05) and significant decrease in all sperm motility, total progressive sperm (A Rapid progressive motility,B Slow or sluggish progressive motility and C Non-progressive motility and D Immotility) and normal sperm morphology in infertile men as compared to fertile control group (P<0.05). Decrease in infertile compared to fertile men was statistically significant (p<0.05), while Cu highly increase in infertile men group compared to fertile group highly significant (p<0.001), decrease in Zn infertile men compared to fertile men highly significant (p<0.001). In addition, decrease in Ni in infertile men as compared to fertile group was highly significant (p<0.001).