Proteomic insight into reduced cell elongation resulting from overexpression of patatin-related phospholipase pPLAIIIδ in Arabidopsis thaliana

Abstract

Patatin-containing phospholipase A (pPLA) hydrolyzes membrane glycerolipids, producing free fatty acids and lysoglycerolipids. Ten pPLAs in the Arabidopsis thaliana genome are grouped into 3 subfamilies, and pPLAIII s differ from pPLAI and II s in their catalytic motifs and overexpression (OE) of pPLAIII s reduces cell elongation and cellulose content. To probe the question of how pPLAIII overexpression results in the changes, comparative proteomic analyses were conducted between pPLAIII δ-OE and WT seedlings. The data indicate a change in the microtubule-associated protein, MA P 18. MA P18 is involved in destabilizing cortical microtubules and modulating directional cell growth. The result suggests that pPLAIII and their derived products may regulate cytoskeletal dynamics leading to retardation in anisotropic growth. © 2014 Landes Bioscience.