Regulation of intracellular pH in hamster preimplantation embryos by the sodium hydrogen (Na+/H+) antiporter

Abstract

This study was an investigation of the mechanisms for the regulation of intracellular pH (pH(i)) by hamster preimplantation embryos. The resting pH values of hamster embryos were similar at the 1-cell (7.19 ± 0.34), 2-cell (7.21 ± 0.21), and 8-cell (7.22 ± 0.41) stages. Cleavage-stage hamster embryos alleviated intracellular acidosis by activity of the Na+/H+ antiporter. The rate of recovery from acidosis was similar for embryos at 1- cell, 2-cell, and 8-cell stages. When Na+/H+ antiporter activity was inhibited by either incubation in Na+-free medium or the presence of an inhibitor, pH(i) was unable to recover to initial levels. Instead, pH(i) remained acidic. The Na+/H+ antiporter was also found to contribute to baseline pH regulation, as incubation in Na+-free medium resulted in an immediate intracellular acidification. The set point for Na+/H+ antiporter was pH 7.14. There was no evidence at any developmental stage for activity of either Na+-dependent HCO3-/Cl- exchanger or H+-ATPase in the regulation of pH(i). Inhibition of the Na+/H+ antiporter by an amiloride derivative significantly reduced the ability of 2-cell embryos to develop in culture when challenged with acidosis, indicating that the Na+/H+ antiporter is an essential regulator of pH(i).