Microsatellite primers in Stipa purpurea (Poaceae), a dominant species of the steppe on the Qinghai-Tibetan Plateau

Abstract

Premise of the study: Microsatellite markers were developed in Stipa purpurea, a dominant species of the steppe and meadow on the Qinghai-Tibetan Plateau. Methods and Results: Using the combined biotin capture method, 15 microsatellite primer sets were isolated and characterized. Eleven of these markers showed polymorphism, and the number of alleles per locus ranged from two to seven across 96 individuals from four populations. Conclusions: These markers provide a useful tool to investigate the spatial genetic structure and mating system of Stipa purpurea. © 2011 Botanical Society of America.