Synthesis, characterization and antimicrobial activity applications of grafted copolymer alginate-g-poly(N-vinyl imidazole)

Abstract

N-Vinyl imidazole was grafted onto sodium alginate (PNVI-g-NaAlg) through a free radical polymerization technique in aqueous solution using potassium persulfate (K2S2O8, KPS) initiator material. The conditions of the grafting process onto sodium alginate were adjusted to obtain a grafted copolymer with a high percentage of poly(N-vinyl imidazole). The prepared grafted copolymer sodium alginate (NaAlg-g-PNVI), with high percentage yield, was investigated and characterized under certain conditions in order to detect its antibacterial effect. The prepared grafted copolymer was considered by means of several systems such as Fourier-Transform Infrared spectroscopy (FT-IR),1H NMR spectroscopy and thermal analysis. The change in the morphology of the alginate distinguished after the grafting process was confirmed using a Scanning Electron Microscope (SEM). The biological activity of the grafted material was considered usingEscherichia coli,Neisseria gonorrhoeae(Gram-negative),Bacillus subtilis(Gram-positive) andCandida albicansantifungal activities through the agar diffusion method. The obtained results show excellent improvement in antimicrobial activity of the alginate by grafting againstBacillus subtilis,Escherichia coli,Neisseria gonorrhoeae, andCandida albicans.