Analysis of production kojic acid from endophytic fungi Aspergillus flavus isolated from Annona squamosa leaves using an OSMAC Approach

Abstract

An analysis production of kojic acid by endophytic fungi Aspergillus flavus from leaves of Annona squamosa has been undertaken using three different types of media including rice (Oryza sativa), sweet corn (Zea mays L.) and waxy corn (Zea mays ceritina). The fungi were extracted using ethyl acetate and quantitatively analyzed their content of kojic acid using HPLC. The intrapolation peak area of kojic acid's content in each fungal medium to the regression equation of standard kojic acid resulted in the concentration of kojic acid in each extract 3.149% for rice media, 5.998% for sweet corn media and 2.226% for waxy corn media. Pure kojic acid from an extract of fungi grown on rice media was obtained from 90% DCM fraction by VLC fractionation. The structure was determined by means of NMR and HPLC analysis data.