The prevalence and genotyping of human papillomavirus in patients with oral tumors in health centers and clinics of Mazandaran in Iran

Abstract

Oral cancer is one of the most prevalent cancers in the world which contains many kinds of malignant neoplasms in the oral cavity. Due to the carcinogenicity of human papillomavirus (HPV) and its prevalence in cancer, including the oral cancer, this study was aimed at investigate the prevalence of HPV and its genotypes in patients suffering from oral tumors using PCR method. In this study, 83 samples of oral lesions were collected in the form of paraffin-embedded tissue. After extracting the DNA using DNA extraction kits, high-risk HPV positive samples were examined using special kits for genotyping, and low-risk types were sequenced after nested PCR. The results showed that 13.2% of samples was HPV positive. The result of PCR using genotyping kit indicated that high-risk types of 18, 31, 16, and 33 appeared in samples with prevalence rate of 27.2, 18.1, 9.09 and 9.09%, respectively. In this manner, the result of sequence indicated that the prevalence of HPV-6 genotype was 36.3% in the samples. The results of this study indicated that both low-risk and high-risk types of HPV are associated with the risk of oral tumors, so that Types 6 and 18 were reported as the most prevalent types in the samples.