Characteristics of alginate content on Sargassum polycystum C.A. Agardh from western Java, Indonesia

Abstract

Utilization of Sargassum polycystum seaweed as an alternative alginate source will reduce dependence on alginate imports, which is currently still 100% imported. Thus, the purpose of this study was to characterize alginates from S. polycystum seaweed obtained from three locations with different ecological characteristics. Alginate isolation by partial hydrolysis separated gulurunic acid (G) and manurunic acid (M) followed by freeze-dried and measured qualitatively and quantitatively using FTIR. A standard curve was made to calibrate the concentration of alginate in each location. The results showed that alginate rendement from S. polycystum of Lima Island, Ujung Kulon, and Binuangeun were 11.48, 18.62, and 5.75%, respectively. The linear regression equation of alginate polymer composition of M/G from Lima Island, Ujung Kulon, and Binuangeun standard curve were y=-14.171x+68.13 R2=0.9242, y=-6.6279x+33.776 R2=0.9811 and y=-9.6763x+59.558 R2=0.9042 respectively. The concentrations of alginate polymers on Lima Island, Ujung Kulon and Binuangeun M/G % were 1.35%, 1.44%, and 2.33%, respectively. It can be concluded that the variations in the concentration of manuronic and guluronic from the three ecologies of S. polycystum in western of Java were different variations.