Selective removal of β2-microglobulin from plasma specimens of long-term hemodialysis patients by high-performance immunoaffinity chromatography

Abstract

We examined the suitability of high-performance immunoaffinity chromatography (HPIAC) for removing β2-microglobulin (β2-MG) from plasma of patients (n = 26) undergoing long-term hemodialysis. We used immobilized antibody to β2-MG to prepare an immunoaffinity column with a 0.5-mL bed volume. Plasma samples of 0.5 mL were chromatographed at a flow rate of 0.5 mL/min and pressures <7 kg/cm2. Although only a small amount of β2-MG can be removed with a conventional dialyzer, 92.1% plusmn; 0.4% of the globulin could be removed by HPIAC. The column could be reused after acid elution. After 50 cycles the efficiency of β2-MG trapping was 73% of the original efficiency.