Protective Еffect of Сranberry Еxtract against Сisрlatin-Induced Neрhrotoxicity by Imрroving Oxidative Stress in Mice

Abstract

Cranberry (Vaccinium macrocarpon) is a North American natural fruit. consumed as food and used for health promotion and prevention of various diseases. The present study was designed to evaluate the protective effect of cranberry fruit extract against nephrotoxicity induced by cisplatin in mice by measuring selected oxidative stress markers. Twenty-eight male albino mice were used in this study. The animals were divided into 4 groups as follows: Grouр I Control/Orally-administered normal saline for 7 successive days; Grouр II [Orally-administered cranberry fruit extract alone (200 mg/kg) for 7 successive days; Grouр III/Mice were orally-administered 0.25ml (0.9% NaCl) for 7 days by gavage tube, and cisplatin at a dose (12mg/kg) were intraperitoneally (IP) injected on day 7 and; Grouр IV [Orally-administered cranberry fruits extract for 7 successive days followed by single IP injection of cisplatin on day 7. After euthanization of each animal by diethyl ether (on day 8th), serum and renal tissue samples were collected for analysis; where the serum was used for assessing urea and creatinine and the renal tissue samples for determining oxidative stress markers (malondialdehyde and reduced glutathione). Administration of cranberry fruit extract resulted in a significant decline in serum creatinine level (0.87±0.120) and a significant elevation in renal reduced glutathione level (197.42±62.958) (P<0.05) with the improvement in the histological analysis of renal tissue of mice of Grouр IV compared to that in cisplatin intraperitoneally-injected Grouр III mice. Orally-administrated cranberry extract prior to cisplatin exerts a protective effect against nephrotoxicity induced by cisplatin via improving the oxidative stress process in mice.