Synthesis of carotenoids by Rhodotorula rubra GED8 co-cultured with yogurt starter cultures in whey ultrafiltrate

Abstract

Two cultures, a yeast (Rhodorula rubra GED8) and a yogurt starter (Lactobacillus bulgaricus 2-11 + Streptococcus thermophilus 15HA), were selected for associated growth in whey ultrafiltrate (WU) and active synthesis of carotenoids. In associated cultivation with the yogurt culture L bulgaricus 2-11 + S. thermophilus 15HA under intensive aeration (1.3 1-1min -1 airflow rate) in WU (45 g lactose 1-1), initial pH 5.5, 30°C, the lactose-negative strain R. rubra GED8 synthesized large amounts of carotenoids (13.09 mg 1-1 culture fluid). The carotenoid yield was approximately two-fold higher in association with a mixed yogurt culture than in association with pure yogurt bacteria. The major carotenoid pigments comprising the total carotenoids were β-carotene (50%), torulene (12.3%) and torularhodin (35.2%). Carotenoids with a high β-carotene content were produced by the microbial association 36 h earlier than by Rhodotorula yeast species. No significant differences were notd in the ratio between the pigments synthesized by R. rubra GED8 + L. bulgaricus 2-11, R. rubra GED8 + S. thermophilus 15HA, and R.rubra GED8 + yogurt culture, despite the fact that the total carotenoid concentrations were lower in the mixed cultures with pure yogurt bacteria. © Society for Industrial Microbiology 2004.