Potensi Ekstrak Daun Sirih dan Rimpang Lengkuas dalam Menekan Pertumbuhan Colletorichum gloeosporiodes Skala In Vitro

Abstract

Colletotrichum gloeosporioides is the cause of anthracnose disease in chili both pre-harvest and post-harvest. Various control efforts continue to be developed to overcome these problems. The purpose of this study was to test the potency of betel leaf extract and galangal rhizome to suppress the growth of C. gloeosporioides on an in vitro scale. This study used a completely randomized design (CRD), consisting of 3 treatments, namely betel leaf extract, galangal rhizome extract, and control. Extracts of betel leaf and galangal rhizome were each prepared at a concentration of 25%, while pure cultures of C. gloeosporioides were isolated from chili peppers with anthracnose symptoms. Then 3 mL of each extract was mixed in 7 mL of Potato Dextrose Agar (PDA) media until homogeneous. Furthermore, the culture of C. gloeosporioides was inoculated on PDA media that had been treated with extract and without extract as a control. The growth diameter of C. gloeosporioides was then observed at 7 days after inoculation. The results showed that the treatment of betel leaf extract and galangal rhizome significantly affected the growth suppression of C. gloeosporioides compared to controls. Galangal rhizome extract was the best treatment in suppressing the growth of C. gloeosporioides with an efficacy of 100%, while betel leaf extract still had a low efficacy of 35%.