MOLECULAR STUDY OF Aeromonas hydrophila ISOLATED FROM STOOL SAMPLES IN NAJAF (IRAQ)

Abstract

The present study included the detection of A. hydrophila in some clinical cases in the governorate of AL-Najaf, during the period from October 2011 to March 2012.The samples was collected from stool samples for A. hydrophila isolates. The PCR method of gene (16S rRNA) was the best methods for diagnosis, which has led to isolate and diagnosis of (23) isolate of A. hydrophila from stool samples. The PCR technique was used for detection of some genes in A. hydrophila like heamolysin (hyl) and aerolysin (aerA) genes which were responsible for pathogenicity of bacteria. The (hyl)and (aerA) genes presented in (80%, 72%) respectively for the clinical isolates.Keywords- Aerolysin and heamolysin genes, 16SrRNA, Aeromonas hydrophila, PCR