Anti-Fungal Activities of Aqueous and Alcoholic Leaf Extracts of Moringa Oleifera Lam. on Candida albicans Isolated from Diabetic Foot Infections

Abstract

Moringa oleifera is a well-known medicinal herb because it has antibacterial and antifungal activity, M.oleifera is A strong source of several phytochemicals that are a drug that's widely used to cure a number of illnesses, such as Anti-Pyretic, Epileptic, Inflammatory disorders Ulcerative, anti-Hypertensive, Cholesterol-lowering, antioxidant, and other medicinal effects. M.oleifera leaf extracts were utilized in the plant component examination and the assessment of the antifungal activity. For experimental purposes, the strain Candida albicans was utilized. To test the anti-Fungal effect of the extracts on fungal cultures, the Well diffusion method was used. The phytochemical screening indicated the presence of (Alkaloids, Flavonoids, Saponins, Steroids, Tannins, Cardiac glycoside, Terpenoids, and the absence of Reducing sugar, Anthraquinones, Phlobatannins) in both extracts. Anti-Fungal activity against Candida albicans was strongly active in Aqueous & Alcoholic Leaf Extracts of Moringa oleifera. Due to the Moringa leaf aqueous extract, an area of inhibition was found from (0.23cm, 0.27, 0.3, 0.39, to 0.58 cm) As there is an increase in concentration from (100 to 500 µg/ml), respectively. While an area It was found that the inhibition induced by ethanolic extract for Moringa leaf increased from (1.29cm,1.40,1.44,1.53,to1.87cm) As there is an increase in concentration from (100 to 500 µg/ml), against Candida albicans, respectively. Nystatin is an antibiotic made of polyene, A section of polyene antifungal compounds, Used for treating fungal infections most commonly. Nystatin has been used against Candida albicans as a positive control, While the region of inhibition appeared (0.5cm) of anti-Nystatin against Candida albicans. This study evaluated various concentrations of extracts Moringa oleifera leaves and anti-Nystatin against Candida albicans. The ethanolic alcoholic extract of Moringa oleifera leaf showed a higher inhibition zone than the aqueous extract and anti-Nystatin.