Identification of cervidae DNA in feedstuff using a real-time polymerase chain reaction method with the new fluorescence intercalating dye EvaGreen

Abstract

A real-time qualitative and quantitative polymerase chain reaction method (cer-194) using the fluorescence dye EvaGreen and aimed at the cytochrome b sequence was established for detection of cervidae DNA in feedstuff. Eight meat meal samples derived from deer, bovine, ovine, camel, pig, rabbit, fish, and chicken and 17 cervidae hair samples covering 2 subfamilies, 4 genera, and 7 species were tested to prove the specificity of the cer-194 system and its universality within the cervidae family. Detection limit of 0.1% deer meat in fish meal, blood powder, and feather powder matrixes was confirmed.