BAR Domain-Containing FAM92 Proteins Interact with Chibby1 To Facilitate Ciliogenesis

Abstract

Chibby1 (Cby1) is a small conserved coiled-coil protein that localizes to centrioles/basal bodies and plays a crucial role in the formation and function of cilia. During early stages of ciliogenesis, Cby1 is required for efficient recruitment of small vesicles at the distal end of centrioles to facilitate basal body docking to the plasma membrane. Here, we identified family with sequence similarity 92, members A and B (FAM92A and FAM92B), which harbor predicted lipid-binding BAR domains, as novel Cby1-interacting partners using tandem affinity purification and mass spectrometry. We found that, in cultured cell lines, FAM92A colocalizes with Cby1 at the centrioles/basal bodies of primary cilia while FAM92B is undetectable. In airway multiciliated cells, both FAM92A and 92B colocalize with Cby1 at the base of cilia. Notably, the centriolar localization of FAM92A and 92B largely depends on Cby1. Knockdown of FAM92A in RPE1 cells impairs ciliogenesis. Consistent with the membrane-remodeling properties of BAR domains, FAM92A and 92B in cooperation with Cby1 induce deformed membrane-like structures containing the small GTPase Rab8 in cultured cells. Our results therefore suggest that FAM92 proteins interact with Cby1 to promote ciliogenesis via regulation of membrane remodeling processes.