Antioxidant capacity of ocimum basilicum L. and Origanum vulgare L. extracts

Abstract

The antioxidant properties of five different extracts (Et 2O, CHCl 3, EtOAc, n-BuOH, and H 2O) of Ocimum basilicum L. and Origanum vulgare L. were studied. Antioxidant activity was assessed in six different model systems. Free radical scavenging capacity (RSC) was evaluated by measuring the scavenging capacity of extracts on DPPH, NO, O 2- and OH radical, as well as on hydrogen peroxide (H 2O 2). In addition, the protective effects on lipid peroxidation in liposomes (LPx) were evaluated by TBA-assay using the Fe 2+/ascorbate induction system. The amount of total phenolic compounds and content of total flavonoids was also determined. EtOAc, n-BuOH and H 2O extracts of O. basilicum and O. vulgare expressed very strong scavenger activity. Furthermore, the mentioned extracts showed notable inhibition of LPx. On the other hand, Et 2O and CHCl 3 extracts showed much weaker effect in the neutralization of DPPH, NO and O 2- radicals and the neutralization of H 2O 2. When examining the production of OH radicals and inhibition of LPx, the Et 2O and CHCl 3 extracts showed weak prooxidative properties. The observed differences in antioxidant activity could be partially explained by the levels of phenolics and flavonoids in the investigated O. basilicum and O. vulgare extracts. © 2011 by The Authors.