Atherosclerosis in C3H/HeJ mice reconstituted with apolipoprotein E-null bone marrow

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Abstract

Previous studies showed that reconstitution of atherosclerosis-susceptible C57BL/6 (B6) female mice with apolipoprotein E (apoE)-deficient (apoE-/-) bone marrow resulted in markedly increased atherosclerosis, despite the fact that plasma lipid levels were unchanged. To determine whether apoE-/- bone marrow would increase atherosclerosis in an atherosclerosis-resistant strain, female C3H/HeJ (C3H) mice were lethally irradiated and reconstituted with bone marrow from either C3H.apoE-/- mice or wild-type C3H mice. Four weeks after transplantation, the mice were fed an atherogenic diet for 12 weeks. We found that reconstitution of C3H mice with apoE-/- bone marrow resulted in a slight reduction in plasma apoE levels and a dramatic reduction in apoE and apolipoprotein B (apoB) in the aortic wall. Plasma apoB and cholesterol levels were unchanged, as were atherosclerotic lesions at the aortic root. These data indicate that reconstitution of C3H mice with apoE-/- bone marrow has no effect on atherosclerosis susceptibility and that apoE promotes accumulation of apoB in the vessel wall.

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Shi, W., Wang, X., Tangchitpiyanond, K., Wong, J., Shi, Y., & Lusis, A. J. (2002). Atherosclerosis in C3H/HeJ mice reconstituted with apolipoprotein E-null bone marrow. Arteriosclerosis, Thrombosis, and Vascular Biology, 22(4), 650–655. https://doi.org/10.1161/01.ATV.0000013388.03553.31

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