Statistical response to different fermentation parameters in rapid production of cellulases by penicillium purpurgenium MA1 in solid state fermentation of rice hulls

Abstract

Time is critical value in biotechnological production of enzymes. Rapid production not only save time and money but also avoid physiological complication of long fungal cultivation. In this study, Plackett-Burman design was employed for screening the effect of 15 nutritional and cultivation parameters in production of CMCase, FPase and β-glucosidase by P. purpurgenium MA1 via solid state fermentation of rice hulls after 30, 36 and 42 h of incubation. The obtained data showed a great variation with different nutrients and with different times. The coefficient of determination (R2) and the adjusted R2 values of the test responses are in reasonable agreement recording ≥0.9. For CMCase, moisture level showed positive effect after 42 h where, pH ranged from negative to slightly positive effect. Inorganic nitrogen sources as (NH4)2SO4 and NH4NO3 have a little positive effect after 30 and 36 h where it recorded high negative effect after 42 h. Yeast extract have a pronounced positive effect in the three tested times. For FPase, Tween 80, pH and CaCl2 have a negative effect in enzyme production. Inorganic nitrogen sources have a positive effect after 42 h in opposite manner and the three organic nitrogen sources have a negative effect. For β-glucosidase, inorganic nitrogen sources have a weak effect as well as peptone where yeast extract have relatively stronger effect, pH have a negative effect after 30 and 36 h, where it have a positive effect one after 42 h. © 2014 Academic Journals Inc.