Antimicrobial activity of enterococcus faecium NM2isolated from urine: Purification, Characterization and bactericidal action of enterocin NM2

Abstract

Cell Free Supernatants (CFS) containing bacteriocin of Enterococcus faecium NM2(E. faecium NM^ isolated from urine inhibited many gram-positive and gram-negative pathogenic bacteria. It also inhibited the Candida albicans M2fungus. The antibiotic sensitivity test of the indicator bacteria showed that these strains were resistant to 60-75% of the antibiotics used. The E. faecium NM2bacteriocin was purified by ammonium sulphate precipitation and gel filtration and a 3600 fold-increase in specific activity of bacteriocin was obtained. The purified bacteriocin showed an apparent molecular mass of Ca, 5 KDa. Amino acid analysis showed that the E. faecium NM2bacteriocin consists of 16 amino acids with high content of glycine, alanine, glutamic acid and asparagine. The E. faecium NM2 bacteriocin was designated enterocin NM2and showed a bactericidal action on sensitive bacterial strains used. Enterocin NM2could be classified as a novel variant within class lie bacteriocins.