Characterization, Phytochemical Screenings and Antioxidant Activity Test of Kratom Leaf Ethanol Extract (Mitragyna speciosa Korth) Using DPPH Method

Abstract

Antioxidants are compounds that have an important role in health because they can be used as anti-toxic molecules in the body which are the cause of various diseases. One of the plants that have antioxidant content is kratom (MitragynaspeciosaKorth). The purpose of this study was to determine the antioxidant activity of kratom leaf ethanol extract by using the DPPH trapping method. Exploration of kratom leaf samples was carried out by maceration using ethanol 96%, macerate was evaporated with a rotary evaporator, phytochemical screening of kratom leaf ethanol extract and antioxidant testing of DPPH as Free radical. Result of Simplisia Characterization of kratom leaves containing water, air soluble extract contents, ethanol-soluble extract levels, total ash content, and acid insoluble ash content sequentially as follows: 6.65; 18.01; 9.45; 7.14; and 1.06%. Phytochemical Screening results containing kratom leaf ethanol extract containing chemical composition: alkaloids, flavonoids, triterpenoids/steroids, saponins, and tannins. The results of antioxidant activity testing showed that ethanol extract had an IC50 value of 38.56 μg / ml. The results showed that the ethanol extract of kratom leaves had antioxidant activity in a very strong category.