10‐Hydroxy‐2‐decenoic Acid, a Major Fatty Acid from Royal Jelly, Inhibits VEGF‐Induced Angiogenesis in Human Umbilical Vein Endothelial Cells

Abstract

Vascular endothelial growth factor (VEGF) is reported to be a potent pro‐angiogenic factor that plays a pivotal role in both physiological and pathological angiogenesis. Royal jelly (RJ) is a honeybee product containing various proteins, sugars, lipids, vitamins and free amino acids. 10‐Hydroxy‐2‐decenoic acid (10HDA), a major fatty acid component of RJ, is known to have various pharmacological effects; its antitumor activity being especially noteworthy. However, the mechanism underlying this effect is unclear. We examined the effect of 10HDA on VEGF‐induced proliferation, migration and tube formation in human umbilical vein endothelial cells (HUVECs). Our findings showed that, 10HDA at 20 µM or more significantly inhibited such proliferation, migration and tube formation. Similarly, 10 µM GM6001, a matrix metalloprotease inhibitor, prevented VEGF‐induced migration and tube formation. These findings indicate that 10HDA exerts an inhibitory effect on VEGF‐induced angiogenesis, partly by inhibiting both cell proliferation and migration. Further experiments will be needed to clarify the detailed mechanism.