Eksplorasi Dosis Efektif Ekstrak Etanol Daun Kipahit sebagai Antipiretik Alami

Abstract

Kipahit plant is one of the plants that have the potential to be used as natural antipyretics. This study was conducted to explore the effective doses of ethanol extract of kipahit leaf (Tithonia diversifolia) as an antipyretics. Twenty four male sprague–dawley white rats weighed150–200 g were divided into 6 groups and replicated 4 times. The experiment included experimental rats without fever induction and without administration of antipyretic substance (control 0), experimental rats with induced fever without administration of antipyretic substance (negative control), experimental rats with induced fever and ethanol extract of kipahit leaf (EEDK) with various doses: 100 mg/kg BW (treatment 1), 200 mg/kg BW (treatment 2), 300 mg/kg BW (treatment 3), and 400 mg/kg BW (treatment 4). Fever was induced by injecting DTP–HB–Hib vaccines intramuscularly at a dose of 0.2 mL/200 g BW. Administration of EEDK was conducted orally at 90 minutes pos-injection of DPT–HB–Hib vaccines. The antipyretic effects of EEDK were observed by measuring rectal temperature by using digital thermometer (correction factor 0.1ºC) in 0 minute (before the injection of DTP–HB–Hib or normal temperature), 90, 120, 150, and 180 minutes post–fever induction. The result showed that the administration of EEDK decreased rectal temperature as compared to negative control. The optimum doses of EEDK administration that decreased rectal temperature were 100 and 200 mg/kg BW. It is concluded that the EEDK has an effect. [MKB. 2017;49(3):139–44]