Identification of genetic susceptibility in preterm newborns with bronchopulmonary dysplasia by whole-exome sequencing: BIVM gene may play a role

Abstract

Bronchopulmonary dysplasia (BPD) is a common chronic respiratory diseasein preterm infants caused by multifactorial etiology. Genetic factors areinvolved in the occurrence of BPD, but studies have found that candidate geneshave poor reproducibility and are influenced by ethnic heterogeneity;therefore, more exploration is still needed. We performed whole-exon sequencingin 34 preterm infants with BPD and 32 non-BPD control neonates. The data wereanalyzed and interpreted by Fisher difference comparison, PLINK and eQTLassociation analysis, KEGG and GO enrichment analysis, STRING tool, Cytoscapesoftware, ProtParam tool, HOPE online software, and GEOR2 analysis on NCBI GEOdataset. BPD has a highly heterogeneity in different populations, and we found35 genes overlapped with previous whole-exon sequencing studies, such as APOBgene. Arterial and epithelial cell development and energy metabolism pathwaysaffect BPD. In this study, 24 key genes were identified, and BIVM rs3825519mutation leads to prolonged assisted ventilation in patients with BPD. A novelDDAH1 mutation site (NM_012137: exon1: c.89 T > G: p.L30R) was found in 9 BPD patients. Conclusion: BIVM gene rs3825519 mutation may play a role in the pathogenesis of BPD by affecting cilia movement, and the DDAH1 and APOB genes mutations may have a pathogenic role in BPD.What is Known:• Genetic factors are involved in the occurrence of bronchopulmonary dysplasia.• The candidate genes have poor reproducibility and are influenced by ethnic heterogeneity, therefore, more exploration is still needed.What is New:• We identified the role of susceptible SNPs in BPD in Shenzhen, China, and identified 24 key genes that influence the pathogenesis of BPD, and also found 35 genes overlapped with previous whole exon sequencing studies, such as APOB gene.• We found that BIVM and DDAH1 genes may play a pathogenic role in the pathogenesis of BPD.