Analysis of the genome of type 7 simian adenovirus using restrictases

Abstract

the effect of the restricting endonucleases R.EcoRI, R.BamI and R.SalI on the genome of type 7 simian adenovirus (SA-7) has been studied. Since the DNA has only one site of R.EcoRI recognition the enzyme cleaves SA-7 DNA into two fragments with the molecular weights 12.0 and l0.0· 106. The restrictase R.BamI cleaves the SA-7 DNA at six sites producing 7 fragments with the molecular weights 6.6, 5.9, 3.8, 2.7, 1.3, 0.7 and 0.6·106. R.SalI cleavage yields 6 fragmentswith the molecular weights 8.1, 5.5, 4.3, 2.45, 1.2 and 0.6·106. The R.BamI and R.SalI fragments are arranged in the orders E-A-D-P-C-G-B and A-B-D-P-E-C, respectively. The only R.EcoRI recognition site is localized in the C fragment produced by R.BamI and in the B fragment produced by R.SalI. © 1978 Information Retrieval Limited.