Development of In Vivo confocal microscope combined with reflection and fluorescence imaging modes

Abstract

We propose a new in vivo confocal microscope capable of reflection and fluorescence imaging simultaneously with the use of 488 nm, 660 nm, and 830 nm lasers. We measured the lateral resolution of less than 0.8μm, the axial resolution of less than 3.5lμ, the focal shift of 1.25μm, and the imaging depth up to 210μm. Ex vivo and in vivo images are acquired from bovine artery endothelial cell, mouse, and human skin tissues. Two imaging modes can provide potentials that the accuracy of diagnosis can be improved from different point of views. © Taylor & Francis Group, LLC.