Economical factitious diet for mass rearing of greater wax moth, Galleria mellonella (Lepidoptera: pyralidae), a promising host for entomopathogenic nematodes

Abstract

Background: Greater wax moth (Galleria mellonella L.) (Lepidoptera: Pyralidae) is most commonly used for mass multiplication of entomopathogenic nematodes. An investigation on economical modification and comparative analysis of factitious diets of G. mellonella was carried out under laboratory conditions. Different diets were assessed in comparison with standard diet. Results: Modifications based on quantity change of dietary components (wheat flour, maize flour, dry milk, dry yeast, honey and glycerin), addition of vitamin E and vitamin-B complexes, elimination of dry milk and replacement of glycerin with sorbitol showed reduction in the cost of production and increase in food conversion efficiency in G. mellonella. The weight of larva in diet III (303.40 mg) was highest as compared to the larval weight in control diet (280.00mg). The conversion of ingested and digested food into biomass of G. mellonella was the highest for factitious diet III. Also, the highest number of Steinernema abbasi was produced from G. mellonella larvae reared on diet III (25418.43IJs) followed by diet I (21805.31IJs). The economics of the diets also showed that the percent cost reduction in factitious diet III and diet I was 48.86 and 28.85%, respectively. Overall, the results suggested that factitious diet III was superior to diet I followed by diet II. Conclusion: Addition of supplements of vitamin E and B-complexes enhances the biological activity of the insect, thereby increasing its food conversion efficiencies. Factitious diet III can be successfully used for mass multiplication of the G. mellonella.