Anti-inflammatory and anti-hyperuricemia properties of chicken feet cartilage: Treatment on gouty arthritis animal model

Abstract

Gout is a form of inflammatory arthritis caused by the deposition of uric acid. The therapeutic approach to gout is mainly divided by the treatment of inflammation and the management of serum urate level. This study aim to investigate whether chondroitin sulfate (CS) and glucosamine in chicken feet cartilage powder (CFE) and aqueous extract (AE) are able to decrease serum urate level and inflammation in animal model of gouty arthritis. CFE and AE were evaluated in vitro for xanthine oxidase (XO) inhibition. The anti-hyperuricemic activity and liver XO inhibition were evaluated in vivo on oxonate-induced hyperuricemia rats. Anti-inflammatory property was also determined on monosodium urate (MSU) crystal-induced paw edema model. CFE and AE supplementation showed urate-lowering activity. However, both treatments were not able to inhibit in vitro and in vivo XO activity. In MSU crystal-induced mice, the levels of paw swelling and lipid peroxidation were increased; in addition, a decrease in the activities of SOD and changes in the expression of CD11b+TNF-α and CD11b+IL-6 of the spleen were demonstrated. These changes were reverted to near normal levels upon CFE and AE treatments. These results suggest that CS and glucosamine from CFE and AE show a potent therapeutic effect against gouty arthritis.