The in vitro effect of zinc on the inhibition of human δ aminolevulinic acid dehydratase by lead

Abstract

The antagonistic effect of zinc on inhibition of human aminolevulinic acid dehydratase (ALAD) by lead was examined in vitro. The phenomenon was studied at pH 6.5 to 7.5. Zinc and lead were added at concentrations ranging from physiological levels to levels far in excess of those expected in heavy industrial exposure. ALAD activity of normal blood assayed in the presence of zinc was unaffected by exogenous lead if the added zinc concentration was about 0.53 mmol/blood. If zinc was added at 0.15 mmol/l blood, the total zinc concentration was approximately 0.28 mmol/blood, or double the normal endogenous level, and was of the order found in heavy industrial exposure to zinc. In this case, ALAD assay values were appreciably raised and might have fallen into the normal range in spite of a dangerous total blood lead of over 0.0048 mmol/l. When zinc was added in vitro to blood from a worker with a blood lead level of 0.0043 mmol/l, the ALAD values obtained were also raised and did not reflect the dangerous level of lead in the patient.