Activation Solutions Effect on Sperm Motility and Fertilization Rates of Oreochromis niloticus

Abstract

Fish sperm-activating media are chemical solutions having certain properties now thought to be a prime indicator for the peculiarity of spermatozoa. Present research evaluated the effect of two laboratoryprepared media and one commercially available media i.e., ACTi fish while control is freshwater on sperm quality parameters i.e., percent motility, motility interval, sustainability, and fertilization rates in Nile tilapia (Oreochromis niloticus). The experimental design was CRD with four treatments and three replicates. Lab prepared (Activation media A and B), one commercially available (Activation media C) and freshwater served as control. After the pre-developed broods were selected based on maturity approaches and collected sperm samples were diluted in the ratio of 1:30 fold media and divided into different aliquots before applying them to in vitro fertilization. It was clear from the results of the current study that, the commercially available activation media C showed a highest significant effect on percent sperm motility i.e., 89.66%±2.88, motility interval 300sec±2.67%, Viability: 74.48±0.39% and fertilization rates: 90.04±1.70% followed by activation media A. motility: 78.24±1.90, interval: 280sec±1.65 viability: 68.21±0.64 and fertilization rates: 78.01±1.81 while B. motility: 68.62±1.08, interval: 250sec.54±0.75%, viability:58.02±0.59 and fertilization rates 70.60±1.02 respectively. The lowest percentage of motility and fertilization rates were recorded in milt aliquots treated with fresh water i.e., control: 53.10±1.02 and 69.60±1.05. Our results indicated, commercially available activation media improved efficiency of sperm motility and fertilization rates.