Dynamics of the DNA damage response: Insights fromlive-cell imaging

Abstract

All organisms have to safeguard the integrity of their genome to prevent malfunctioning and oncogenic transformation. Sophisticated DNA damage response mechanisms have evolved to detect and repair genomic lesions.With the emergence of live-cell microscopy of individual cells, we now begin to appreciate the complex spatiotemporal kinetics of the DNA damage response and can address the causes and consequences of the heterogeneity in the responses of genetically identical cells.Here, we highlight key discoveries where live-cell imaging has provided unprecedented insights into howcells respond to DNA double-strand breaks and discuss themain challenges and promises in using this technique. © The Author 2013. Published by Oxford University Press. All rights reserved.