Ambient particulate matter affects occludin distribution and increases alveolar transepithelial electrical conductance

Abstract

Background and objective: Inhaled particulate matter (PM) causes lung inflammation and epithelial dysfunction. However, the direct effect of PM on alveolar epithelial barrier integrity is not well understood. Our aim is to determine whether PM exposure affects the alveolar epithelial cells (AEC) transepithelial electrical conductance (Gt) and tight junction (TJ) proteins. Methods: Human AEC (A549) and primary rat AEC were exposed to PM of <10 A ;μm in size (PM 10) and diesel exhaust particles (DEP), using titanium dioxide (TiO 2) as a control for particle size effects. Gt and permeability to fluorescein isothiocyanate-dextran (FITC-Dextran) were measured to assess barrier integrity. TJ integrity was evaluated by analysing penetration of Lanthanum nitrate (La 3+) under transmission electron microscopy. Surface proteins were labelled with biotin and analysed by western blot. Immunofluorescence was performed to assess colocalization of TJ proteins including occludin and zonula occludens-1 (ZO-1). PM induced dissociation of occludin-ZO-1 was evaluated by co-immunoprecipitation. Results: PM 10 and DEP increased Gt and disrupted TJ after 3 h of treatment. PM 10 and DEP induced occludin internalization from the plasma membrane into endosomal compartments and dissociation of occludin from ZO-1. Overexpression of antioxidant enzymes manganese superoxide dismutase (MnSOD) and catalase, prevented PM-induced Gt increase, occludin reduction from the plasma membrane and its dissociation from ZO-1. Conclusions: PM induces alveolar epithelial dysfunction in part via occludin reduction at the plasma membrane and ZO-1 dissociation in AEC. Furthermore, these effects are prevented by overexpression of two different antioxidant enzymes. This work studies the effects of particulate matter and diesel exhaust particles on alveolar barrier integrity in vitro. Our results show that these particles alter tight junction integrity, specifically the occludin and ZO-1 association. These effects are prevented by blocking of mitochondrial ROS production, suggesting a central role of this organelle in the effects observed. © 2011 Asian Pacific Society of Respirology.