Effects of Semi-Static Exposure to Carbofuran in Liver Phase I and Phase II Enzymes of Common Carp (Cyprinus carpio)

Abstract

The effect of semi-static exposition of common carp (Cyprinus carpio L) to different concentrations (0, 10, 50 and 100 μg/L) of the pesticide carbofuran has been evaluated. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) and benzyloxy-4-[trifluoromethyl]-coumarin-O-debenzyloxylase (BFCOD) activities, as a phase I biotransformation parameters; and (2) uridine diphospho-glucuronosyltransferase (UDPGT) activity as phase II conjugation enzyme. A clear inhibition of both EROD and BFCOD activities was observed in fish exposed to carbofuran with respect to controls, whereas in the case of UDPGT activity, values of exposed animals showed a clear increase during the experiment with respect to controls.