Lipid-soluble vitamins from dairy products: Extraction, purification, and analytical techniques

Abstract

Milk and dairy products are considered as essential sources of lipid-soluble vitamins (LSVs) for human nutrition. Due to the lower concentrations, complexity, and instability of LSVs during extraction, their quantification remains challenging. This review focus on advances in the extraction and quantification of LSVs from different dairy products. Saponification, and liquid-liquid (LLE), solid-phase (SPE), and supercritical fluid (SFE) extraction methods, as well as dispersive liquid-liquid microextraction, are the most common techniques. Liquid chromatography-mass spectrophotometry (LC-MS) has unique advantages for LSVs determination and quantification due to its high sensitivity and specificity.