In vitro development of pig preantral follicles.

Abstract

A limiting factor to realizing the full potential of many of the new reproductive techniques is the lack of abundant numbers of fertilizable oocytes. This problem could be addressed by using the large source of oocytes available from preantral and primordial follicles by developing systems for in vitro growth. In vitro systems that use early growing follicles as a source of oocytes have been developed for laboratory species and these have been successful in producing live young. If successful, in vitro growth in association with in vitro maturation (IVM) and cryopreservation would optimize in vitro production systems. In vitro growth systems that support the growth of pig preantral follicles have been developed and have been successful in producing meiotically competent oocytes but, to date, no live young have been produced. However, these systems remain to be characterized and their main application is as experimental models to study the processes of early oocyte and follicle development. This review provides an overview of culture systems that have been developed for domestic species and discusses how these are furthering our basic knowledge of early follicular development, as well as considering the benefits and potential problems associated with in vitro growth systems.