Intrauterine administration of bacterial modulins : An alternative therapy for endometritis

Abstract

To study recovery of endometritis in cows following intrauterine administration of certain bacterial modulins prepared in laboratory, twenty-four cows with endometritis were randomly divided into four groups of six animals each. The animals of group-1 were given 100 μg lipopolysaccharide (LPS) of E. coli (Sigma Chemicals Co., USA), group-II animals received LPS of E. coli isolated from endometritis cows in laboratory (ILPS) using same dose and group-HI bacteria free filtrate (BFF) of Staphylococcus aureus isolated from endometritis cows in the laboratory on the day of estrus. The control group animals received saline at similar times. There was a significant increase in total leucocyte count and the polymorphonuclear cells. The levels of immunoglobulins were higher in groups of animals that received LPS preparations but not in BFF-treated animals. A significant (P<0.05) reduction in bacterial load was observed in all treatment groups at subsequent estrus. The overall conception rate tended to be higher in group-I as compared to group-II and III. This study demonstrated therapeutic efficacy of laboratory preparations of LPS of E. coli and BFF of Staphylococcus aureus in endometritis stimulating the mechanisms of uterine defense as evident by increased influx of PMN cells and immunoglobulin levels in uterine fluid of treated animals. © GSP, India.